引物设计保护碱基列表

引物设计保护碱基列表保护碱基列表%CleavageChainEnzymeOligoSequenceLength2hr20hrGGTCGACC800AccICGGTCGACCG1000CCGGTCGACCGG1200CACATGTG800AflUICCACATGTGG109090CCCACATGTGGG129090GGCGCGCC89090AsciAGGCGCGCCT109090TTGGCGCGCCAA129090CCCCGGGG85090AvalCCCCCGGGGG109090TCCCCCGGGGGA129090CGGATCCG81025BamlllCGGGATCCCG109090CGCGGATCCGCG129090CAGATCTG800BglllGAAGATCTTC107590GGAAGATCTTCC122590GGCGCGCC800BssHIIAGGCGCGCCT1000TTGGCGCGCCAA125090BstEIIGGGTA/TACCC9010AACTGCAGAACCAATGCATTGG2200BstXIAAAACTGCAGCCAATGCATTGGAA242550CTGCAGAACCAATGCATTGGATGCAT272590CATCGATG800GATCGATC800ClalCCATCGATGG109090GGAATTCC89090EcoRICGGAATTCCG109090CCGGAATTCCGG129090GGGGCCCC89090HaelllAGCGGCCGCT109090TTGCGGCCGCAA129090CAAGCTTG800HindlllCCAAGCTTGG1000CCCAAGCTTGGG121075GTTTAAAC800GGTTTAAACC10025PmelGGGTTTAAACCC12050AGCTTTGTTTAAACGGCGCGCCGG247590GCTGCAGC800TGCACTGCAGTGCA141010PstIAACTGCAGAACCAATGCATTGG229090AAAACTGCAGCCAATGCATTGGAA249090CTGCAGAACCAATGCATTGGATGCAT2600CCGATCGG800PvulATCGATCGAT101025TCGCGATCGCGA12010SacICGAGCTCG81010GCCGCGGC800SacIITCCCCGCGGGGA125090GTCGACGTCAAAAGGCCATAGCGGCCGC2800SallGCGTCGACGTCTTGGCCATAGCGGCCGCGG301050ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA321075GAGTACTC81025SealAAAAGTACTTTT127575CCCGGG6010CCCCGGGG8010SmalCCCCCGGGGG101050TCCCCCGGGGGA129090GACTAGTC81090GGACTAGTCC101090SpelCGGACTAGTCCG12050CTAGACTAGTCTAG14050GGCATGCC800SphICATGCATGCATG12025StulGAAGGCCTTC109090AAAAGGCCTTTT129090CTCTAGAG800GCTCTAGAGC109090XbalTGCTCTAGAGCA127590CTAGTCTAGACTAG147590CCTCGAGG800XholCCCTCGAGGG101025CCGCTCGAGCGG121075CCCCGGGG800CCCCCGGGGG102575XmalCCCCCCGGGGGG125090TCCCCCCGGGGGGA149090。